The present invention relates to an anticancer composition containing an active ingredient consisting of GKN 1, and more specifically relates to an anticancer composition containing an active ingredient consisting of GKN 1 protein or an expression vector comprising the GKN 1 gene which has an outstanding anticancer activity. The GKN 1 according to the present invention is advantageous in that it can beneficially be used as a composition for anticancer therapy because it features not only outstanding activity in suppressing the occurrence of cancer cells but also outstanding activity in suppressing cancer cell metastasis during over-expression.