The present invention provides a prosthetic heart valve comprising a stent, an alloy wire and biological tissue leaflets, the biological tissue leaflets being sewn to the alloy wire which is wrapped with a first polyester cloth, and the alloy wire being sewn to the stent which is wrapped with a second polyester cloth, wherein the stent is ring-shaped, comprising: a first stent layer and a second stent layer which are closely hooping with each other the first stem layer being on the outside of the second stent layer, the first stent layer being made of a first material, the second stent layer being made of a second material, the first material and the second material being elastic, and the hardness of the second material being higher than that of the first material. Through the design of making the stent always tend to remain annular both in a stationary state and in a stressed state, the prosthetic heart valve of the present invention ensures the perfect match between the stent and the alloy wire, thereby increasing the stability of the entire valve and extending the service life of the valve.