The present invention is for a vaccine comprising of recombinant protein t2544 of Salmonella typhi (a recombinant polypeptide sequence (SEQ ID 1), and a modified recombinant polypeptide sequence (SEQ ID 2) ) which confers cell mediated immunity against Salmonella typhi. Further, the vaccine comprising of recombinant protein t2544 of Salmonella typhi of SEQ ID 1 or 2 is emulsified with adjuvants, wherein the adjuvant be Freund"s complete adjuvant or Freund"s incomplete adjuvant.