KOSMEDER, Jerome,LEFEVER, Mark,JOHNSON, Donald,FARRELL, Michael,ZHILINA, Zhanna,BIENIARZ, Christopher
申请号：
ES20160151995T
公开号：
ES2663080(T3)
申请日:
2007.11.01
申请国别(地区):
西班牙
年份:
2018
代理人:
摘要：
A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels. In particular, in this invention the haptens are a thiazole sulfonamide or a benzofurazan. The linker in the conjugate is a polymer comprising 1 to 15 ethylene glycol units. A kit with a hapten and anti-hapten
