The invention provides the cell colony that comprises the part mature dendritic cell that can be used for the individual administration of tumor.With about 10 hours of about 1-of dendritic cell maturation reagent effect or the sophisticated dendritic cell of part more of a specified duration, can effectively absorb and the processing tumor antigen at tumor locus, and full maturity, can migrate to the individual lymph node place of treatment subsequently.In case in the arrival lymph node, the suitable cytokine (for example TNF α and IL-12) of full ripe angtigen presentation dendritic cell secretion is induced follow-up anti-tumor immune response with the T cytosis.
