A two-photon imaging system capable of imaging of an image region in real time is presented. The imaging system comprises a source of excitation light that provides the excitation light as a plurality of laser beamlets. The plurality of laser beamlets is collectively scanned by a single-axis scanner along a first direction in the focal plane of the image region and oriented such that neither the rows nor columns are aligned with the first direction. As a result, each laser beamlet scans a different sub-region of the image region and the plurality of sub-regions are simultaneously scanned. As a result, the entirety of the image region is scanned in the same amount of time required to scan one image sub-region.
