Embodiments of the present application provide a headphone with a biological feature detection function, an interaction system and a biological feature detection method. The headphone includes: a loudspeaker, a microphone, a biological feature detection module configured to detect a biological feature of a user, a mode switching module configured to switch, under control of a control module, to determine whether the headphone is in a working mode supporting a biological feature detection mode, a first communication module, a control module and a power management module configured to supply power to modules in the headphone. The control module is configured to detect and determine whether an instruction is received from the smart terminal, and control, according to the instruction received from the smart terminal, the biological feature detection module, the mode switching module, the power management module and the first communication module to work, and further configured to parse the biological feature from the biological feature detection module under control of the instruction received from the smart terminal,, and transmit the parsed biological feature to the smart terminal via the first communication module. The headphone according to the embodiments of the present application is capable of automatically implementing the biological feature detection function.
