The present application provides a method for generating chloroplast gene modified plant cells, comprising: constructing a pair of nuclear gene modifying vectors； transforming the pair of nuclear gene modifying vectors into plant cells viaAgrobacterium； and selecting and culturing the plant cells. The nuclear gene modifying vectors comprises a promoter, a recombinant gene conferred the chloroplast transit peptide and TALEN protein domain, a terminator as well as right border and left border of T-DNA regions. Thus, by using nuclear transformation to mutate chloroplast gene, the problems such as low transformation efficiency, long selection time, labor-intensive and high cost encountering in chloroplast transformation would be avoided.本申請案提出一種產生葉綠體基因變異的轉殖植物細胞的方法，包括：建構一對前述之細胞核基因轉殖載體；透過農桿菌將所述一對細胞核基因轉殖載體併入植物細胞；以及篩選及培養植物細胞。細胞核基因轉殖載體包括啟動子、葉綠體訊息蛋白基因、重組TALEN蛋白域、終結子以及T-DNA的右邊界區及左邊界區。如此一來利用細胞核基因轉殖達到造成植物葉綠體基因變異的目標，能夠避免傳統葉綠體基因轉殖效率低、篩選時間長、耗費人力及物力等問題並達到相同目標。
