Subhayu Basu,Gaozhong Shen,Brett Adam Boghigian,David M. Young,David Arthur Berry,Geoffrey von Maltzahn
申请号：
US14335071
公开号：
US20150057352A1
申请日:
2014.07.18
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and α-linolenic acid.
