B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG
发明人:
Frank Niehaus,Jürgen Eck,Renate Schulze,Michael Krohn
申请号：
US15879737
公开号：
USRE047528E1
申请日:
2018.01.25
申请国别(地区):
US
年份:
2019
代理人:
摘要：
We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease； (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3； (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80% identical to the amino acid sequence of (a), preferably at least 85% identical, more preferably at least 90% identical, and most preferred 95% identical； (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80% identical to the nucleotide sequence of (b), preferably at least 85% identical, more preferably at least 90% identical, and most preferred 95% identical； (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d)； or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.
