A composition for preventing or treating inflammatory diseases, comprising an extracellular vesicle derived from lactic acid bacteria as an active ingredient
The present invention relates to a composition for the prevention, amelioration, or treatment of an inflammatory disease comprising an extracellular vesicle derived from lactic acid bacteria isolated from a culture solution of lactic acid bacteria as an active ingredient, and a method for diagnosing atopic dermatitis. The extracellular vesicles derived from lactic acid bacteria according to the present invention are significantly more distributed in the urine and blood of patients with inflammatory diseases than healthy subjects, and inflammation of epithelial cells and inflammatory cells by exposure of extracellular vesicles derived from staphylococci or Pseudomonas bacteria Since it was confirmed through in vitro and in vivo experiments that the reaction was efficiently suppressed by extracellular vesicles derived from lactic acid bacteria, the composition containing extracellular vesicles derived from lactic acid bacteria as an active ingredient was atopic dermatitis, chronic Expected to be useful in the development of pharmaceuticals, cosmetics or health functional foods to prevent, ameliorate, or treat inflammatory diseases such as rhinitis, chronic sinusitis, asthma, chronic obstructive pulmonary disease, or sepsis Is done. It may also be useful for diagnosing atopic dermatitis by measuring the distribution of extracellular vesicles derived from lactic acid bacteria from urine or blood samples. [Selection] Figure 32本発明は乳酸菌の培養液から分離した乳酸菌由来の細胞外ベシクルを有効成分として含む炎症疾患の予防、改善、または治療用の組成物、およびアトピー性皮膚炎の診断方法に関するものである。本発明による乳酸菌由来の細胞外ベシクルは健常者比べて炎症疾患患者の尿および血液に顕著に多く分布されており、ブドウ球菌またはシュードモナス菌由来の細胞外ベシクルの露出による上皮細胞および炎症細胞の炎症反応が乳酸菌由来の細胞外ベシクルにより効率的に抑制されることをｉｎ ｖｉｔｒｏおよびｉｎ ｖｉｖｏ実験を通じて確認したので、上記の乳酸菌由来の細胞外ベシクルを有効成分として含む組成物はアトピー性皮膚炎、慢性鼻炎、慢性副鼻腔炎、喘息、慢性閉塞性肺疾患、または敗血症などの炎症疾患を予防、改善、または治療するための医薬品、化粧品、または健康機能食品の開発に有用に用いることができると期待される。また、尿または血液サンプルから乳酸菌由来の細胞外ベシクルの分布を測定することによりアトピー性皮膚炎を診断するのにも有用に用いることができるだろう。【選択図】図３２
