A construction method for an immunodeficient rat model. The method comprises the following steps: performing in-vitro transcription on Prkdc of rat DNA, and gRNA having IL2Rγ gene knocked out, respectively mixing with in-vitro transcribed CAS9 protein mRNA respectively freezing in an RNA enzyme-free ultrapure water to obtain corresponding injections A and B performing amplification and purification on human SIRPa genomic DNA and freezing in an RNA enzyme-free ultrapure water to obtain an injection C using a microinjection method, respectively injecting the injections A and B into the cytoplasm of different rat zygotes, and injecting the injection C into the pronucleus of the other rat zygote implanting the three zygotes into different pseudopregnant females for cultivating to obtain second-generation rats A, B and C respectively performing hybridization on the second-generation rats A, B and C to reproduce F1 generation and performing hybridization on the F1 generation to obtain the immunodeficient rat model in which the IL2Rγ and Prkdc genes are knocked out and the human SIRPa gene is transferred. Human cells can be effectively implanted into the rat model the model is in accordance with text indexes and strain background is pure.Linvention concerne une méthode de construction dun modèle de rat immunodéficient. La méthode comprend les étapes suivantes : réalisation dune transcription in vitro de la Prkdc de lADN de rat, et de lARNg ayant un gène IL2Rγ inactivé, respectivement mélange avec lARNm de protéine CAS9 transcrit in vitro respectivement congélation dans une eau ultrapure exempte denzyme dARN pour obtenir des injections correspondantes A et B réalisation dune amplification et dune purification de lADN génomique SIRPa humain et congélation dans une eau ultrapure exempte denzyme dARN pour obtenir une injection C utilisation dune méthode de micro-injection, respectivement injection des injections A et B dans le cytoplasme de différents zygotes de rat, et inject
