The present invention provides a water outlet sterilization device of a water dispenser and a water dispenser. The sterilization device includes: a housing, a water inlet channel formed in the housing, and a sterilization module provided in the housing; the water inlet channel is in communication with the water outlet of the water dispenser ; The sterilization module includes a sterilization cylinder and a sterilization lamp assembly sealed on the top of the sterilization cylinder. The top side wall of the sterilization cylinder is provided with a water inlet communicating with the water inlet channel. The bottom end of the sterilization cylinder is equipped with a flow guide, The bottom end is provided with a water outlet hole, and the water entering the sterilization cylinder is sterilized by the germicidal lamp assembly, and then flows out through the water outlet hole. The invention installs a sterilization device downstream of the water outlet of the drinking fountain. The water enters and exits through the sterilization device. The water from the outlet enters the sterilization barrel through the top of the sterilization barrel, and then flows downward under the action of gravity, and finally is collected by the guide The process of the water outlet hole flowing through the quartz glass and the inside of the sterilization cylinder is continuously illuminated by the sterilization lamp assembly, which improves the aseptic effect of the water outlet of the drinking fountain.本發明提供一種飲水機出水口殺菌裝置及飲水機,該殺菌裝置包括:殼體、形成於殼體內的進水通道、及設於殼體內的殺菌模組;進水通道與飲水機出水口連通;殺菌模組包括殺菌筒和密封設於殺菌筒頂端的殺菌燈組件,殺菌筒的頂部側壁設有與進水通道連通的進水孔,殺菌筒的底端安裝導流件,導流件的底端設有出水孔,進入殺菌筒內的水由殺菌燈組件進行殺菌後,經出水孔流出。本發明在飲水機出水口下游安裝殺菌裝置,水通過殺菌裝置上進下出,出水口流出的水先經殺菌筒的頂部進入殺菌筒,再經重力作用往下流動,最後由導流件匯集至出水孔流出,流過石英玻璃和殺菌筒內部的過程不斷被殺菌燈組件照射,提高了飲水機出水的無菌效果。