An isolator system 1 includes: a main isolator 3 in which an aseptic state is maintained and which is for performing an aseptic operation； an incubator 4 in which the aseptic state is maintained and which is connected to the main isolator 3 and is for culturing cells and the like； decontamination means 35 for decontaminating the inside of the main isolator 3； and a decontamination station 9 that decontaminates the inside of the incubator 4. The isolator system 1 further includes a blocking member 30 for sealing a connection port 13 from the outside, the port being provided in the main isolator 3 and being for connection with the incubator 4. When the inside of the main isolator 3 is decontaminated, the connection port 13 is sealed from the outside with the blocking member 30. The isolator system that can efficiently decontaminate the main isolator and the subisolator connected to this main isolator can be provided.隔離系統1包括：主隔離器3，內部維持為無菌狀態而用以進行無菌作業；恆溫箱4，內部維持為無菌狀態且連接於主隔離器3，而用以培養細胞等；去污染手段35，將主隔離器3之內部去污染；以及去污染台9，將恆溫箱4之內部去污染；隔離系統1中進而包括封鎖構件30，該封鎖構件30用以自外部將設置於主隔離器3之用以連接恆溫箱4之連接埠口13密閉。於將主隔離器3之內部去污染之情形時，利用封鎖構件30自外部將連接埠口13密閉。本發明可提供能夠分別有有效率地將主隔離器、及連接於該主隔離器之副隔離器去污染之隔離系統。
