The present invention relates to a method for producing lymphocytes including NK cells, the capability of producing interferon-γ of which is significantly enhanced, by culturing mononuclear cells in the presence of gamma globulin. In particular, the present invention relates to a method for producing lymphocytes including activated natural killer cells for targeting cancer cells that express HER2/neu antigen such as breast cancer and ovarian cancer, by binding an anti-HER2/neu antibody to a lymphocyte having activated natural killer cells, and also relates to a pharmaceutical composition including same.La présente invention concerne un procédé pour produire des lymphocytes comprenant des cellules NK, dont la capacité de production d'interféron γ est significativement augmentée, par culture de cellules mononucléaires en présence de gamma-globuline. En particulier, la présente invention concerne un procédé pour produire des lymphocytes comprenant des cellules tueuses naturelles activées pour cibler des cellules cancéreuses qui expriment un antigène HER2/neu tel que le cancer du sein et le cancer ovarien, par liaison d'un anticorps anti-HER2/neu à un lymphocyte comprenant des cellules tueuses naturelles activées, et concerne en outre une composition pharmaceutique comprenant ceux-ci.본 발명은 감마글로불린 존재하에서 단핵구 세포를 배양함으로써 인터페론-γ 생성능이 현저하게 증가된 NK 세포를 포함하는 림프구의 제조방법을 제공한다. 또한, 본 발명은 활성화된 자연살해 세포를 포함하는 림프구에 항-HER2/neu 항체를 결합시킴으로써, 유방암, 난소암 등의 HER2/neu 항원을 발현하는 암세포로의 표적지향을 위한 자연살해 세포를 포함하는 림프구의 제조방법 및 이를 포함하는 암세포 표적지향을 위한 약학 조성물을 제공한다.
