PROBLEM TO BE SOLVED: To provide three-dimensional cell cultured matter containing a blood vessel-like hollow tubular structure therein.SOLUTION: The three-dimensional cell cultured matter is prepared by a method containing a process (a) for manufacturing a core shell type gel fiber containing the dissociated gel fiber of a core part, the cell adhesive gel of a shell part and a blood vessel constructing cell, a process (b) for culturing the gel fiber to obtain a core-shell type gel fiber containing the cultured matter of the blood vessel constructing cell in the cell adhesive gel of the shell part, a process (c) for obtaining a hollow blood vessel-like structure by removing the dissociated gel fiber of the core part and a process (d) for obtaining the three-dimensional cell cultured matter containing the hollow blood vessel-like structure therein by embedding the hollow blood vessel-like structure by a tissue constructing cell to perform culture.COPYRIGHT: (C)2012,JPO&INPIT【課題】血管様の中空管状の構造物を内部に含む三次元細胞培養物を提供する。【解決手段】(a)コア部の解離性ゲルファイバ及びシェル部の細胞接着性ゲルを含み、血管構築用細胞を含むアシェル型ゲルファイバを製造する工程;(b)該ゲルファイバを培養し、シェル部の細胞接着性ゲルに血管構築用細胞の培養物を含むコアシェル型ゲルファイバを得る工程;及び(c)コア部の解離性ゲルファイバを除去して中空の血管様構造物を得る工程;及び(d)該血管様構造物を組織構築用細胞で包埋して培養を行ない、中空の血管様構造物を内部に含む三次元細胞培養物を得る工程を含む方法。【選択図】図1
