The present invention provides a single cancer direct detection device comprises an electrode having a nanopore biomimetic membrane inducing a bio-communication signal by cancer cells selectively under antibody-free and label-free condition. Methods of detecting cancer cells and monitoring cancer progresses in a contour map format are also discussed. In particular, the cancer cell heat release map comprises multiple variables correlations, a ratio of “Action potential/Resting potential” (RAPRP) as a special biomarker for the variable along with other variables of current or concentration makes visual display cancer progresses possible compared with normal cells which have negligible heat release.
