Raymond E.B. Ketchum,Colby G. Caldwell,Sung-Yong H. Yoon
申请号：
US14996411
公开号：
US20160208299A1
申请日:
2016.01.15
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Natural product preparations of isoquinoline alkaloids are obtained from plant callus cells of goldenseal, Hydrastis canadensis, grown in suspension cell culture. The natural product preparations can be extracted from the cells and/or obtained from the culture medium in which the cells are grown. Accordingly, callus cell lines can be selected to grow in suspension culture, produce desirable levels and/or ratios of isoquinoline alkaloids, and/or release the isoquinoline alkaloids into the liquid medium. The level and/or ratio of certain isoquinoline alkaloids, such as hydrastine and/or berberine produced by the cells and/or released into the medium can be different than those found in native plants or plant parts (e.g., rhizomes) from which the cells were derived. For instance, cell lines can be selected to increase the production and/or ratio of hydrastine to berberine, or vice versa.
