Recombinant construct, recombinant microorganism, recombinant plant cell and method of providing plant with resistance against DNA virus and RNA virus

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专利权人:

NATIONAL CHUNG HSING UNIVERSITY

发明人:

Yeh Shyi-Dong,Yang Ching-Fu,Chen Kuan-Chun,Huang Ya-Ling

申请号：

US201514802614

公开号：

US10000766(B2)

申请日:

2015.07.17

申请国别(地区):

美国

年份:

2018

代理人:

Muncy, Geissler, Olds & Lowe, P.C.

摘要：

Provided is a strategy for generating transgenic plants with concurrent resistance to DNA and RNA viruses at one construction, so as to develop an RNA-directed DNA methylation (RdDM) transgenic system using a hairpin construct of Ageratum yellow vein virus (AYVV) promoter region residing in an intron to resist DNA virus infection by RdDM. Furthermore, the hairpin construct of the AYVV promoter region coupled with an untranslatable nucleocapsid protein (NP) fragment of Melon yellow sport virus (MYSV) is created to induce post-transcriptional gene silencing (PTGS) against MYSV. A method for providing transgenic plants conferring concurrent resistance to both AYVV and MYSV for control of DNA and RNA virus at the same time, and underlying RdDM and PTGS mechanisms, respectively, is also provided.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/