A device to prepare and deliver a bioactive substance such as stem cells, platelet rich plasma, and/or other bioactive compositions. The device can utilize a hallow interior or cavity to deliver bioactive substances during a surgical procedure or to an area of cartilage defect or cartilage disorder. The treated tissues can include connective tissue, cardiac muscle or tissue, spinal tissue, internal organs, skin tissue, brain tissue, vascular tissue, ocular, ear, nose, throat tissue, and/or other hematologic, endocrine or integumentary tissue.
