Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Moskovskij gosudarstvennyj universitet imeni M.V. Lomonosova" (MGU)
发明人:
Kopeina Gelina Sergeevna,Zamaraev Aleksej Vladimirovich,Lavrik Inna Nikolaevna,Zhivotovskij Boris Davidovich
申请号：
RU20160126059
公开号：
RU2671510(C1)
申请日:
2016.06.29
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: proposed group of inventions relates to medicine. Methods for isolating a high molecular weight protein complex for the activation of human caspase-2, formed in cancer cells in response to DNA-damaging chemotherapeutic treatment, are proposed. Cancer cells are incubated in the presence of a DNA-damaging chemotherapeutic drug until the maximum level of caspase-2 activation is obtained. Cells are harvested, followed by addition of a lysis buffer, separation of the cell debris from the lysate containing the high-molecular caspase-2 activation complex, separation by gel filtration of the resulting cell lysate, with the separation of fractions containing high-molecular forms of caspase-2 from fractions containing its monomeric forms and isolation from the fraction containing high-molecular forms of caspase-2, a high-molecular protein complex of caspase-2 activation.EFFECT: proposed group of inventions provides an effective isolation of the complex of activation of human caspase-2.36 cl,
