The inventions relate to an apparatus for aligning and positioning pieces of food dough with predetermined orientations and at predetermined intervals, and provide an aligning and positioning apparatus that can easily adjust positions of the pieces of food dough in a perpendicular direction to a moving direction of the pieces and that can process at high speed. An aligning and positioning apparatus of the inventions includes a holding unit having a plurality of holding mechanisms to hold a plurality of pieces of food dough carried by a first conveyor, a driving mechanism of the holding mechanisms, guide members to control the positions of the holding mechanisms in the perpendicular direction when the holding mechanisms are moved by the driving mechanism, and a positioning mechanism to adjust positions of the guide members in the perpendicular direction.