The present invention relates to a kind of methods, for (104) (102) in test sample. The method comprising the steps of for particle: reading measuring signal (115) indicates the attribute in magnetic field that at least one is detected from an interface (110) at least one magnetic field sensor (130) at least through a sub-quantity sample (102). In the method, sample (102) are equipped with magnetic detection particle (106) and are designed to that (104) in conjunction with particle detect. This method further comprises the steps of: determining cymomotive force, the characteristic and step at least one magnetic field of particle (106) are detected depending on two, determine that at least one detection particle (106) of bonding state is used as unbonded or is bonded to particle (104), it is the intensity to be detected according to determining fluctuation, in order to detect the particle (102) in (104). Sample
