A system for imaging an abnormal cell and or diseased tissue after topical application of a near infrared protease-specific probe is disclosed. The system includes a protease-specific near infrared (NIRF) imaging probe, means for topically administering the protease-specific probe to a diseased tissue and an imaging device capable of detecting the interaction between the protease-specific NIRF imaging probe and the diseased tissue. A method and surgical procedure are also disclosed.
