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MODIFICATION OF RECOMBINANT Adenovirus by Immunogenic Epitopes of Protein CIRCUMSPOROZOITE PLASMODIA
专利权人:
ДЗЕ РОКФЕЛЛЕР ЮНИВЕРСИТИ (US)
发明人:
СИРАЦУТИ Такаюки (JP),ЦУДЗИ Мория (US)
申请号:
RU2012110255/10
公开号:
RU2012110255A
申请日:
2010.08.18
申请国别(地区):
RU
年份:
2013
代理人:
摘要:
1. Recombinant adenovirus derived from a plasmid vector based on a recombinant adenovirus that contains a nucleotide sequence encoding: a) a plasmodium circumsporozoite (Plasmodium) protein gene or its antigenic part, operably linked to a heterologous promoter sequence, and b) one or more modified protein genes a capsid and / or core, wherein a plasmodium circumsporozoite plasmodium immunogenic epitope sequence has been integrated into at least a portion of one or more capsid protein genes and / or c rdtseviny or replaces said chast.2. The adenovirus according to claim 1, wherein the circumsporozoite Plasmodium protein gene further comprises a circumsporozoite Plasmodium falciparum or Plasmodium yoelii protein gene. The adenovirus according to claim 1, wherein the plasmodium circumsporozoite protein gene further comprises a circumsporozoite protein gene Plasmodium falciparum or Plasmodium yoelii with optimized codons. The adenovirus according to claim 3, wherein said optimized codon protein gene is encoded by the sequence of SEQ ID NO: 1 or SEQ ID NO: 2.5. The adenovirus according to claim 1, wherein said immunogenic epitope sequence further comprises a plasmodium circumsporozoite protein epitope sequence recognized by B lymphocytes and / or T lymphocytes. The adenovirus according to claim 5, wherein said capsid protein gene further comprises a sequence of a hypervariable hexon region (HRV). The adenovirus according to claim 6, wherein the HRV sequence further comprises an HRV1 or HRV5 sequence and an epitope recognized by B lymphocytes: a) is inserted into the HVR1 or HVR5 sequence, or b) replaces part of the HVR1 or HVR5.8 sequence. Adenovirus according to claim 7, in which the modified white gene1. Рекомбинантный аденовирус, полученный из плазмидного вектора на основе рекомбинантного аденовируса, который содержит последовательность нуклеотидов, кодирующую:а) ген белка circumsporozoite плазмодия (Plasmodium) или его антигенной части, функционально связанный с посл
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