The manner which produces the granulation die culture bone where as for this invention, yield is high, is superior in bone regenerative probability is offered. While production manner of the granulation die culture bone which depends on this invention with the culture solution entering the mesenchyme type main stem cell which was cultured to the primary culture process which cultures the mesenchyme type main stem cell the marrow liquid which was picked from the patient, by culturing the cell which is derived from the periosteum and the fat etc inside the nutrient medium and the deep bottom container where the porous pseudo bone granulation is beforehand entered, making the aforementioned porous pseudo bone granulation put in afloat temporarily the seeding process which it seeds and the mesenchyme type main stem cell which is seeded with the porous pseudo bone granulation by culturing inside the nutrient medium the culture bone bud cell way to the cellIt features that the differentiation culture process which it differentiates is had.本発明は、収率が高く、骨再生確率に優れた顆粒型培養骨を製造する方法を提供する。本発明にかかる顆粒型培養骨の製造方法は、患者から採取した骨髄液、骨膜、脂肪等に由来する細胞を培地内で培養することにより間葉系幹細胞を培養する一次培養工程と、多孔質擬似骨顆粒があらかじめ投入された深底容器に培養された間葉系幹細胞を培養液とともに投入し、前記多孔質擬似骨顆粒を一時的に浮揚状態とさせつつ播種する播種工程と、播種された間葉系幹細胞を多孔質擬似骨顆粒とともに培地内で培養することにより培養骨芽細胞様細胞へ分化させる分化培養工程を備えることを特徴とする。
