(57)< Abstract > This invention offers the hybrid polypeptide which raises MHC class II antigen presentation. The said hybrid, II key peptide LMRKLPKPPKPVSKMR of the mammal (arrangement number 1) and including the alteration ones which keep the activity which raises the antigen, the N end which becomes, including antigen epitopu of form of the polypeptide or the peptide imitation structure which it connects to the region which bends the antigen peptide of the MHC class II molecule, has the inclusion chemical structure which C end, and N end and C terminal component which become it shares connects. And you describe also the identification method of the molecule which functioning is done inside circumstance of the hybrid which raises MHC class II antigen presentation with the style which is equal to II key peptide. In addition as for this invention, it offers also the method of raising the presentation of antigen epitopu which is limited to the MHC class II to T cell. It offers in vivo and ekusubibo both method. And it offers also the identification method of the chemical compound which obstructs MHC class II antigen presentation.本発明は、MHCクラスII抗原提示を高めるハイブリッドポリペプチドを提供する。該ハイブリッドは、哺乳動物のIi keyペプチドLMRKLPKPPKPVSKMR(配列番号1)および抗原を高める活性を保持するその改変物を含んで成るN末端、MHCクラスII分子の抗原ペプチドを曲げる部位に結合するポリペプチドもしくはペプチド模倣構造の形態の抗原エピトープを含んで成るC末端、ならびにN末端およびC末端成分を共有結合する介在化学構造を有する。Ii keyペプチドと同等な様式でMHCクラスII抗原提示を高めるハイブリッドの情況内で機能する分子の同定方法もまた記述する。本発明はまた、T細胞へのMHCクラスIIに限定される抗原エピトープの提示を高める方法も提供する。インビボおよびエクスビボ双方の方法を提供する。MHCクラスII抗原提示を阻害する化合物の同定方法もまた提供する。
