#$%^&*AU2017203004A120171130.pdf#####ABSTRACT A method of operating a sterilization system having a vacuum chamber for sterilizing instruments, the chamber connected to a reservoir of sterilant by a valve in a closed state, is disclosed. The method comprises placing the instruments in a non-sterile state in a sterilization pack, opening the chamber, placing the pack into the chamber, closing the chamber, withdrawing a first volume of air from the chamber, changing a volume of liquid water into vapor, opening the valve, introducing the sterilant into the chamber, withdrawing the sterilant from the chamber, introducing a second volume of air into the chamber, opening the chamber, removing the pack from the chamber, and removing the instruments in a sterile-state from the pack. The method may also include taking a baseline humidity measurement while the pressure within the chamber is a first pressure, lowering the pressure within the chamber to a conditioning pressure, maintaining the conditioning pressure for a dwell time, increasing the pressure within the chamber, acquiring a second humidity measurement from within the chamber, and comparing the baseline humidity measurement to the second humidity measurement.1/5 FIG. 1 10 50 3852 12 28 18 20 22 Pump 3 26 Load 33 35 F Load V V 36 24 40 30 3 2 44 2 --Processor Sterilant Reservoir lStcrage iiTimer 38 42 45 28