Lewis, Jennifer A.,Skylar-Scott, Mark A.,Kolesky, David B.,Homan, Kimberly A.,Ng, Alex H.m.,Church, George M.
申请号:
AU2016226178
公开号:
AU2016226178A1
申请日:
2016.03.03
申请国别(地区):
AU
年份:
2017
代理人:
摘要:
Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.