A system may include an ultrasound probe and a controller unit configured to communicate with the ultrasound probe. The controller unit may be further configured to transmit ultrasound signals using the ultrasound probe toward an area of interest in a patient's body, wherein the ultrasound signals include a fundamental frequency signal and at least one harmonic frequency signal； receive echo signals from the area of interest based on the transmitted ultrasound signals； obtain a fundamental frequency echo signal and at least one harmonic frequency echo signal from the received echo signals； and generate a visual representation of the area of interest based on the obtained fundamental frequency echo signal and the obtained at least one harmonic frequency echo signal.
