Disclosed herein are methods of producing an acellular organ. The method includes the steps of, subjecting an organ derived from an animal to a static supercritical fluid (SCF) treatment followed by a dynamic SCF treatment. Optionally, the method of the present disclosure further includes a hypertonic and a hypotonic treatments prior to the static SCF treatment, and/or a neutralizing treatment after the dynamic SCF treatment. Also disclosed herein are acellular organs produced by the present method. The present acellular organs are devoid of any cellular matter, and thus may server as a bio-scaffold for host cells to grow thereon after transplantation without eliciting undesired rejection responses.本揭示內容是關於用以製備去細胞器官的方法。本發明方法包含對源自動物的器官施以靜態超臨界流體處理後，再施以動態超臨界流體處理。非必要地，本揭示內容之方法更包含在靜態超臨界流體處理前施以一高張及低高處理，以及／或是在動態超臨界流體後施以一中和處理。本揭示內容亦提供以本發明方法製備的去細胞器官。本發明之去細胞器官不具有任何細胞物質，因此移植後可作為支持宿主細胞生長的生物骨架，而不會引發不良的排斥反應。
