A fabricating device of three-dimensional scaffolds includes a bubble generating device, a bubble mixing channel, a coagulating solution channel and a bubble collector. The bubble generating device includes a gas channel, a gel solution channel and a separating body. The gel solution channel is around the gas channel. The separating body is disposed between the gas channel and the gel solution channel. The bubble mixing channel connects to a first outlet of the bubble generating device. The coagulating solution channel connects to the bubble mixing channel. The bubble collector connects to a second outlet of the bubble mixing channel.一種三維支架之製備裝置,包括一氣泡產生器、一氣泡混合通道、一凝結溶液通道以及一氣泡收集器。氣泡產生器包括一氣體流道、一膠體溶液流道及一隔離體。膠體溶液流道係環繞氣體流道。隔離體係設置於氣體流道與膠體流道之間。氣泡混合通道係與氣泡產生器之一第一出口連接。凝結溶液通道係與氣泡混合通道連接。氣泡收集器係與氣泡混合通道之一第二出口連接。1‧‧‧製備裝置11‧‧‧氣泡產生器111‧‧‧氣體流道112‧‧‧膠體溶液流道114‧‧‧第一出口12‧‧‧氣泡混合通道121‧‧‧彎折部122‧‧‧第二出口13‧‧‧凝結溶液通道14‧‧‧氣泡收集器2‧‧‧氣流供應單元3‧‧‧膠體溶液供應單元4‧‧‧凝結溶液供應單元A-A‧‧‧剖面線S‧‧‧距離
