Федеральное государственное бюджетное учреждение науки Государственный научный центр Российской Федерации - Институт медико-биологических проблем Российской академии наук (ГНЦ РФ - ИМБП РАН) (RU)
发明人:
Буравкова Людмила Борисовна (RU),Андреева Елена Ромуальдовна (RU),Андрианова Ирина Вячеславовна (RU),Горностаева Александра Николаевна (RU),Сотнезова Елена Викторовна (RU),Григорьев Анатолий Иванович
申请号：
RU2016104419
公开号：
RU2016104419A
申请日:
2016.02.10
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: invention relates to production of cell cultures enriched with hematopoietic precursor cells with CD34/CD133phenotype. The method includes preparation of a stromal sublayer, addition of an umbilical cord fraction, cultivation and selection. Multipotent stromal cells of adipose tissue (MSC) are obtained from the stromal-vascular fraction of adipose tissue, which are cultured at an Oconcentration of 5% in the environment. Mononuclear fraction of umbilical cord blood (MNF UB) is added to the MSC monolayer. After cocultivation, the undifferentiated hematopoietic precursors are selected from the mononuclear fraction of UB by adhesion to MSC at 20% O, MSC is continued to be cultured with attached MNF UB for 96 hours at Oconcentration of 20% in the environment.EFFECT: invention allows to obtain a population enriched with undifferentiated hematopoietic umbilical cord blood precursors with this complex phenotype.9 dwg, 1 tbl, 1 ex
