UNIWERSYTET ROLNICZY IM. HUGONA KOŁŁĄTAJA W KRAKOWIE
发明人:
MAŁGORZATA MALIK,ANNA BACH
申请号：
PL414981
公开号：
PL414981A1
申请日:
2015.11.27
申请国别(地区):
PL
年份:
2016
代理人:
摘要：
fragments of and rate of ovarian abortion narcissus \"carlton\", of a thickness of 1 - 2 mm, carved with flower buds isolated from onions, diameter 12 cm shall be affixed in accordance with the direction of growth on solid medium with a ph of 5.5, composed of makroelement\u00f3w and minerals and sucrose, auksyny,...... okininy and agar. culture is without light, at a temperature of 18 - 22\u00b0c at 4 to 6 week transit.after manufacture nodularnych kalus\u00f3w primary somatic embryos and transferring them to fresh medium of the same composition as medium inicjalna and maintains for a further 12 - 18 weeks, until the conversion of primary somatic embryos in homogeneous soft kalusy about k remowym colour.then the kalusy separates from eksplantat\u00f3w primary and carries on the namna\u017caj\u0105c\u0105 composition identical with medium inicjaln\u0105 and leads their multiplication in subsequent repeated 4 - 6 weekly cycles, by covering the following 2.5 - 5 g of tissue kalusowej on szalk\u0119 pe triego.then in order to regenerate embryos tissue kalusow\u0105 moves on medium of ph 5.8 regenerative fascia containing makroelementy and micronutrients and auksyn\u0119 sucrose, naa, ba and cytokinin\u0119 agar and handles culture in access light at 18 - 22\u00b0c in such a waythat the regenerative fascia teaches after 5 - 10 g tissue kalusowej on 1 szalk\u0119 petri dish and gradually during the passage of tissue separates obtained plants, resulting from the conversion of somatic embryos. the regeneration phase includes at least 3 - 4 6-week-old passages.Fragmenty pędów i zalążni narcyza Carlton o grubości 1 - 2 mm, wycięte z pąków kwiatowych izolowanych z cebul o średnicy 12 cm umieszcza się zgodnie z kierunkiem wzrostu na pożywce stałej o pH o wartości 5,5, złożonej z makroelementów i mikroelementów oraz sacharozy, auksyny, cytokininy i agaru. Kulturę prowadzi się bez dostępu światła, w temperaturze 18 - 22°C przy 4 - 6 tygodniowym pasażu. Po wytworzeniu nodularnych
