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Molecules and methods for protein inhibition and detection
专利权人:
VIB VZW;Vrije Universiteit Brussel;Katholieke Universiteit Leuven K.U. Leuven R&D;Universiteit Gent
发明人:
SCHYMKOWITZ, Joost,ROUSSEAU, Frederic
申请号:
ES12707813
公开号:
ES2679995T3
申请日:
2012.03.12
申请国别(地区):
ES
年份:
2018
代理人:
摘要:
A method for inducing the aggregation of a protein comprising contacting said protein with a molecule of the following structure: (X2i-1-Yi-X2i-Zi) n, wherein: - n is an integer from 1 to 5 ei increases by 1 an with each repetition; - each of X2i-1 and X2i is independently selected from 1 to 4 contiguous amino acids selected from: R, K, E, D, P, N, S, H, G and Q; more particularly, 1 to 4 amino acids selected from R, K, E, D and P; - each Yi is an aggregating beta sequence independently selected from a series of 6 to 16 contiguous amino acids, at least 50% of these hydrophobic amino acids and in which at least one aliphatic moiety or F is present and in case only an aliphatic moiety or F is present, at least one and preferably at least two, different moieties are selected from Y, W, A, M and T; and in which no more than 1 and preferably no residue of P, R, K, D or E is present and where at least one Yi is an identical series or that differs in no more than one amino acid substitution from a series contiguous amino acids found in said protein; and - each Zi is a linker and Zn is independently selected from a linker or nothing; wherein the molecule is capable of inducing the aggregation of said protein; and where the method is not practiced in the body of a human being or an animal.Un método para inducir la agregación de una proteína que comprende poner en contacto dicha proteína con una molécula de la siguiente estructura: (X2i-1-Yi-X2i-Zi)n, en donde: - n es un número entero de 1 a 5 e i aumenta de 1 a n con cada repetición; - cada uno de X2i-1 y X2i se selecciona independientemente de entre 1 a 4 aminoácidos contiguos seleccionados de entre : R, K, E, D, P, N, S, H, G y Q; más particularmente, 1 a 4 aminoácidos seleccionados de entre R, K, E, D y P; - cada Yi es una secuencia beta agregante seleccionada independientemente de entre una serie de 6 a 16 aminoácidos contiguos, siendo al menos un 50% de estos aminoácidos hidrofóbicos y en la que está presente al menos u
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