There are disclosed systems and methods for non-invasive delivery of an agent to biological tissues. Delivery of the agent to the tissues is by ultrasound and delivery induces an immune response in the subject. In some embodiments the systems and methods use agent carrier body including a tissue contacting surface for non-invasively engaging tissues under treatment. The tissue contacting surface can be at least partly defined by a plurality of protrusions that are in fluid communication with one or more reservoirs forming part of the agent carrier body. The protrusions may extend outward from an inside of a void and terminate at said tissue contacting surface.
