Miriam Tendler,Celso Raul Romero Ramos,Andrew J. G. Simpson
申请号：
US14886794
公开号：
US09475838B2
申请日:
2015.10.19
申请国别(地区):
US
年份:
2016
代理人:
摘要：
A process for purification of a fatty acid binding proteins such as, e.g., Sm14 of Pichia pastoris or type-3 FABP protein of Fasciola hepatica. The process includes the steps of: (a) performing lysis of cells containing the fatty acid binding protein to obtain a lysate； (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate； (c) loading the clarified lysate in a column containing an anion exchange resin； (d) eluting proteins from the column by pH changes in the column； and (e) separating contaminant proteins from the fatty acid binding protein by gel-filtration.
