Metallic zinc and the ratio of cadmium are significant to the error free proliferation of cell and differentiation. A possibility that understanding effect of the cadmium in cancer disease due to and provide a possibility that being best understood from cancer, and preventing, treating and/or alleviate cancer. The reliability of the cell or tissue culture method of product or ovum of Zn (II) concentration for using the serum or comprising serum in tire serum, the human serum from Cord blood or healthy donors and the Poultry Serum including complete ovum and ovum product and repeatable significance. Disclosed herein is the method and steps that a kind of manufacture serum product is notably intended to the serum product for the test of in vitro culture or tissue, wherein measures the concentration of Zn (II) and is preferably adjusted to desired section. Also disclose the different product being made of serum or serum component or comprising serum or serum component.