A method of manufacturing a medical product having an engineered heparin bioactive matrix for clinical application on a blood contacting surface comprises: a) activating a blood contacting surface of at least one component of a medical device via one of plasma treatment or gas activation； b) assembling the medical product； c) Setting up medical device for wet chemistry in which wet chemistry treatments follows a blood flow path through device； d) enhancing at least the blood contacting surface with a wet chemistry treatment including an aqueous solution having a strong oxidizing agent, such as ammonium persulfate； e) adding a positively charged spacer molecule to at least the blood contacting surface with a wet chemistry treatment including an aqueous solution having a cationic polymer, such as PEI； and f) covalently immobilizing heparin to at least the blood contacting surface with a wet chemistry treatment including heparin, preferably deaminated heparin.一種製造供臨床應用在血液接觸表面上具有經工程處理肝素生物活性基質之醫學產品的方法，其包含：a)經由電漿處理或氣體活化之一活化一醫學裝置之至少一個組件的血液接觸表面；b)組裝該醫學產品；c) 設立醫學裝置用於濕化學，其中濕化學處理遵循通過該裝置的血液流動路徑；d) 以濕化學處理增強至少該血液接觸表面，該濕化學處理包括具有強氧化劑的一水溶液，諸如過硫酸銨；e)以濕化學處理將一帶正電荷的間隔分子(spacer molecule)加入到至少該血液接觸表面，該濕化學處理包括具有一陽離子聚合物的一水溶液，諸如PEI；及f) 以包括肝素的濕化學處理，將肝素共價固定到至少該血液接觸表面上，較佳地脫胺肝素。
