Institute of Microbiology; Chinese Academy of Sciences
发明人:
Wenjun Liu,Weinan Zheng,Jing Li
申请号：
US16085469
公开号：
US10538745B2
申请日:
2017.01.22
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A recombinant virus is obtained by mutating a codon that encodes a tyrosine residue at position 385 of NP protein in the genome of influenza A virus to a codon of phenylalanine residue. The virus WSN-Y385F is a temperature-sensitive virus that can normally replicate and survive at 37° C., and cannot normally replicate and cannot survive at 33° C. Phosphorylation of a NP protein of influenza A virus can be inhibited by mutating an amino acid residue at position 385 from N terminal of the NP protein of influenza A virus, from a tyrosine to a phenylalanine. The recombinant virus can be used in analyzing mechanisms of infection by influenza virus, and in connection with methods of prevention and treatment of infection by influenza virus.
