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COSMID VECTOR FOR PLANT TRANSFORMATION AND USE THEREOF
专利权人:
发明人:
申请号:
EP07767526.2
公开号:
EP2042602B1
申请日:
2007.06.25
申请国别(地区):
EP
年份:
2012
代理人:
摘要:
The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that:1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups2) they contain the trfA1 gene of an IncP plasmid3) they contain an oriT of an IncP plasmid4) they contain the incC1 gene of an IncP plasmid5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA6) they contain a drug resistance gene expressed in E.coli and a bacterium of the genus Agrobacterium7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant and10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.
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