The invention of a production device for queen bee and royal jelly comprises a spawn-incubating unit 20 including spawn-incubating plates 21, 22, worker bee inlets 23 and a queen bee inlet 24 on the plates 21, 22, spawning holes 25 formed on a side of the inlets 23, 24 in which the queen bee can spawn, and feed chambers 32 formed as separate chambers at a side of the inner space 28 and communicating with the inner space 28 or formed on the inner side of the plates 21, 22 as a plurality of recesses, in which feeds such as pollen, water and sugar solution are supplied a plurality of larva cell pieces 41 or a larva cell strip 40 and a production unit 50 which includes a plurality of queen houses 60 or a queen house strip 52.
