Disclosed herein is a probe assembly, an ultrasound imaging apparatus and a controlling method the ultrasound imaging apparatus.The ultrasound imaging apparatus includes a placing unit in which at least one probe connector is placed, an identification information receiver receiving probe assembly identification information from the placed probe connector, an input device receiving a selection command of at least one probe connector, and a locking unit locking the at least one probe connector with the ultrasound imaging apparatus based on the selection command.