Beilinson Vadim,Jones Janice,Parks Jessica,Thayer Rebecca E.,Tomso Daniel J.,Uknes Scott Joseph,Volrath Sandy,Ward Eric Russell
申请号：
US201815862184
公开号：
US2018135045(A1)
申请日:
2018.01.04
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental mic
