A biological information acquisition apparatus includes a light emitting part provided on a detection surface facing a measurement site and irradiating the measurement site with a non-laser illumination light, a laser irradiation part provided on the detection surface and irradiating the measurement site with a laser, a light receiving part provided on the detection surface and receiving lights from the measurement site, and generating a first detection signal indicating light reception intensity when the illumination light is radiated and a second detection signal indicating light reception intensity when the laser is radiated, an irradiation control unit that controls whether or not to allow laser irradiation by the laser irradiation part according to the first detection signal, and an analytical processing unit that acquires biological information according to the second detection signal.