An improved vaccine for immunization of waterfowl such as ducks and geese comprises an inactivated strain of a mycoplasma infecting waterfowl, such as Mycoplasma sp. strain 1220 the vaccine can include an excipient and an adjuvant. Methods for immunization of waterfowl with the vaccine are also described. The present invention is based, in part, on successful isolation and characterization of mycoplasmas present in geese and ducks and selection of suitable, immunologically active strains of waterfowl mycoplasma, such as the 1220 type of mycoplasma.Linvention concerne un vaccin amélioré pour limmunisation de sauvagine, telle que des canards et des oies, comprenant une souche inactivée dun mycoplasme infectant une sauvagine, telle que la souche 1220 de Mycoplasma sp. le vaccin peut comprendre un excipient et un adjuvant. Linvention concerne également des procédés dimmunisation de sauvagine à laide du vaccin.