#$%^&*AU2015258323B220170601.pdf#####Abstract The present invention relates to a method of producing a collagen membrane that has particular mechanical properties. In particular, the present invention relates to a method A of producing a collagen membrane comprising the steps of (i) isolating a collagen-containing tissue and incubating same in an ethanol solution； (ii) incubating the collagen-containing tissue from step (i) in a first solution comprising an inorganic salt and an anionic surfactant in order to denature non-collagenous proteins contained therein； (iii) incubating the collagencontaining tissue produced in step (ii) in a second solution comprising an inorganic acid until the collagen in said material is denatured； and (iv) incubating the collagen-containing tissue produced in step (iii) in a third solution comprising an inorganic acid with simultaneous mechanical stimulation for sufficient time to enable the collagen bundles in said collagencontaining tissue to align； wherein the mechanical stimulation comprises applying tension cyclically to the collagen-containing tissue. 7145822 1 (GHMatters) P90666.AU.2 KAROLA
