Anke Rattenholl;Adelbert Grossmann;Elisabeth Schwarz;Rainer Rudolph
发明人:
Anke Rattenholl,Adelbert Grossmann,Elisabeth Schwarz,Rainer Rudolph
申请号：
US09807096
公开号：
US08318671B1
申请日:
1999.10.11
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.
