PROBLEM TO BE SOLVED: To provide a method for creating a mammal nuclear transplantation embryo, relatively conveniently, and efficiently.SOLUTION: A mammal nuclear transplantation embryo is cultured in a culture medium containing a histone deacetylation enzyme inhibitor and a deionized serum albumin, and thereafter, the mammal nuclear transplantation embryo is transferred into a culture medium containing vitamin C and a deionized serum albumin and cultured there.SELECTED DRAWING: NoneCOPYRIGHT: (C)2018,JPO&INPIT【課題】哺乳動物核移植胚を、比較的簡便に、かつ効率よく作出する方法を提供すること。【解決手段】哺乳動物の核移植胚を、ヒストン脱アセチル化酵素阻害剤と脱イオン化血清アルブミンとを含む培養液中で培養し、その後、哺乳動物の核移植胚を、ビタミンＣと、脱イオン化血清アルブミンとを含む培養液中に移し、培養する。【選択図】なし
