System for detecting the level of discomfort of aquatic animals during experimental studies, with a water tank, a reference electrode placed at one of the sides of the tank, at least one recording electrode placed at another side of the tank, a bio amplifier for amplifying a received bio-signal at the recording electrode, a microprocessor for treating the signals and a low-pass filter for filtering the signals received. The system can provide a precise, quantifiable and specific indication of the level of stress/wellbeing of aquatic animals in normal living conditions as well as in experimental conditions without interfering with the animals life.